ecl western blot analysis system Search Results


ecl western blot analysis system  (Revvity)
99
Revvity ecl western blot analysis system
Ecl Western Blot Analysis System, supplied by Revvity, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ecl western blot analysis system/product/Revvity
Average 99 stars, based on 1 article reviews
ecl western blot analysis system - by Bioz Stars, 2026-02
99/100 stars
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western blot analysis kit amersham ecl plus  (Amersham Life Sciences Inc)
90
Amersham Life Sciences Inc western blot analysis kit amersham ecl plus
CB1 and TRPV1 protein–protein interaction. (A) Anti-TRPV1 antibody selectivity validation. <t>Western</t> <t>blot</t> detects TRPV1 protein expression with apparent molecular weight in agreement with previous reports. It was selectively suppressed by 74% decline following TRPV1 siRNA transfection of HCEC. (B) CB1-induces decline in TRPV1 phosphorylation status. Western blot <t>analysis</t> of TRPV1 immunopreciptiates using an anti-phospho-threonine antibody revealed that 30 min 10 μM WIN preincubation dampened TRPV1 phosphorylation. Anti-phosphothreonine antibody specificity was validated based on blot with the positive control provided by the commercial source. (C) Anti-CB1 antibody selectivity validation. Western blot detects CB1 protein expression with apparent molecular weight in agreement with previous reports. It was selectively suppressed by 76% decline following CB1 siRNA transfection. (D) TRPV1 complexation with CB1 and TAK1. Co-immunoprecipitation of whole cell lysates obtained with either an anti-TAK1, CB1 or TRPV1 antibody following CAP (10 μM) exposure. Immunocomplexes were probed for TRPV1 content with Western blot analysis using an anti-TRPV1 antibody. (E) CB1 complexation with TRPV1 and TAK1. Co-immunoprecipitation of whole cell lysates obtained with either an anti-TAK1, CB1 or TRPV1 antibody following CAP (10 μM) exposure. Immunocomplexes were probed for CB1 content with Western blot analysis using an anti-CB1 antibody. Antibody selectivity was validated by showing that the IgG immunoprecipitated complexes yielded no immunoreactive bands. Results are expressed as means±SEM (n=3). Differences of p<0.05 were considered as significant.
Western Blot Analysis Kit Amersham Ecl Plus, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/western blot analysis kit amersham ecl plus/product/Amersham Life Sciences Inc
Average 90 stars, based on 1 article reviews
western blot analysis kit amersham ecl plus - by Bioz Stars, 2026-02
90/100 stars
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ecl western blot analysis system containing peroxidase-labeled anti-mouse or anti-rabbit antibodies  (Amersham Pharmacia Biotech Ltd)
90
Amersham Pharmacia Biotech Ltd ecl western blot analysis system containing peroxidase-labeled anti-mouse or anti-rabbit antibodies
CB1 and TRPV1 protein–protein interaction. (A) Anti-TRPV1 antibody selectivity validation. <t>Western</t> <t>blot</t> detects TRPV1 protein expression with apparent molecular weight in agreement with previous reports. It was selectively suppressed by 74% decline following TRPV1 siRNA transfection of HCEC. (B) CB1-induces decline in TRPV1 phosphorylation status. Western blot <t>analysis</t> of TRPV1 immunopreciptiates using an anti-phospho-threonine antibody revealed that 30 min 10 μM WIN preincubation dampened TRPV1 phosphorylation. Anti-phosphothreonine antibody specificity was validated based on blot with the positive control provided by the commercial source. (C) Anti-CB1 antibody selectivity validation. Western blot detects CB1 protein expression with apparent molecular weight in agreement with previous reports. It was selectively suppressed by 76% decline following CB1 siRNA transfection. (D) TRPV1 complexation with CB1 and TAK1. Co-immunoprecipitation of whole cell lysates obtained with either an anti-TAK1, CB1 or TRPV1 antibody following CAP (10 μM) exposure. Immunocomplexes were probed for TRPV1 content with Western blot analysis using an anti-TRPV1 antibody. (E) CB1 complexation with TRPV1 and TAK1. Co-immunoprecipitation of whole cell lysates obtained with either an anti-TAK1, CB1 or TRPV1 antibody following CAP (10 μM) exposure. Immunocomplexes were probed for CB1 content with Western blot analysis using an anti-CB1 antibody. Antibody selectivity was validated by showing that the IgG immunoprecipitated complexes yielded no immunoreactive bands. Results are expressed as means±SEM (n=3). Differences of p<0.05 were considered as significant.
Ecl Western Blot Analysis System Containing Peroxidase Labeled Anti Mouse Or Anti Rabbit Antibodies, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ecl western blot analysis system containing peroxidase-labeled anti-mouse or anti-rabbit antibodies/product/Amersham Pharmacia Biotech Ltd
Average 90 stars, based on 1 article reviews
ecl western blot analysis system containing peroxidase-labeled anti-mouse or anti-rabbit antibodies - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
tm ecl western blot analysis substrate  (Promega)
90
Promega tm ecl western blot analysis substrate
CB1 and TRPV1 protein–protein interaction. (A) Anti-TRPV1 antibody selectivity validation. <t>Western</t> <t>blot</t> detects TRPV1 protein expression with apparent molecular weight in agreement with previous reports. It was selectively suppressed by 74% decline following TRPV1 siRNA transfection of HCEC. (B) CB1-induces decline in TRPV1 phosphorylation status. Western blot <t>analysis</t> of TRPV1 immunopreciptiates using an anti-phospho-threonine antibody revealed that 30 min 10 μM WIN preincubation dampened TRPV1 phosphorylation. Anti-phosphothreonine antibody specificity was validated based on blot with the positive control provided by the commercial source. (C) Anti-CB1 antibody selectivity validation. Western blot detects CB1 protein expression with apparent molecular weight in agreement with previous reports. It was selectively suppressed by 76% decline following CB1 siRNA transfection. (D) TRPV1 complexation with CB1 and TAK1. Co-immunoprecipitation of whole cell lysates obtained with either an anti-TAK1, CB1 or TRPV1 antibody following CAP (10 μM) exposure. Immunocomplexes were probed for TRPV1 content with Western blot analysis using an anti-TRPV1 antibody. (E) CB1 complexation with TRPV1 and TAK1. Co-immunoprecipitation of whole cell lysates obtained with either an anti-TAK1, CB1 or TRPV1 antibody following CAP (10 μM) exposure. Immunocomplexes were probed for CB1 content with Western blot analysis using an anti-CB1 antibody. Antibody selectivity was validated by showing that the IgG immunoprecipitated complexes yielded no immunoreactive bands. Results are expressed as means±SEM (n=3). Differences of p<0.05 were considered as significant.
Tm Ecl Western Blot Analysis Substrate, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tm ecl western blot analysis substrate/product/Promega
Average 90 stars, based on 1 article reviews
tm ecl western blot analysis substrate - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
western blot (immunoblot) analysis ecl  (Amersham Life Sciences Inc)
90
Amersham Life Sciences Inc western blot (immunoblot) analysis ecl
CB1 and TRPV1 protein–protein interaction. (A) Anti-TRPV1 antibody selectivity validation. <t>Western</t> <t>blot</t> detects TRPV1 protein expression with apparent molecular weight in agreement with previous reports. It was selectively suppressed by 74% decline following TRPV1 siRNA transfection of HCEC. (B) CB1-induces decline in TRPV1 phosphorylation status. Western blot <t>analysis</t> of TRPV1 immunopreciptiates using an anti-phospho-threonine antibody revealed that 30 min 10 μM WIN preincubation dampened TRPV1 phosphorylation. Anti-phosphothreonine antibody specificity was validated based on blot with the positive control provided by the commercial source. (C) Anti-CB1 antibody selectivity validation. Western blot detects CB1 protein expression with apparent molecular weight in agreement with previous reports. It was selectively suppressed by 76% decline following CB1 siRNA transfection. (D) TRPV1 complexation with CB1 and TAK1. Co-immunoprecipitation of whole cell lysates obtained with either an anti-TAK1, CB1 or TRPV1 antibody following CAP (10 μM) exposure. Immunocomplexes were probed for TRPV1 content with Western blot analysis using an anti-TRPV1 antibody. (E) CB1 complexation with TRPV1 and TAK1. Co-immunoprecipitation of whole cell lysates obtained with either an anti-TAK1, CB1 or TRPV1 antibody following CAP (10 μM) exposure. Immunocomplexes were probed for CB1 content with Western blot analysis using an anti-CB1 antibody. Antibody selectivity was validated by showing that the IgG immunoprecipitated complexes yielded no immunoreactive bands. Results are expressed as means±SEM (n=3). Differences of p<0.05 were considered as significant.
Western Blot (Immunoblot) Analysis Ecl, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/western blot (immunoblot) analysis ecl/product/Amersham Life Sciences Inc
Average 90 stars, based on 1 article reviews
western blot (immunoblot) analysis ecl - by Bioz Stars, 2026-02
90/100 stars
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ecl western blot analysis kit 93-k820-500  (MultiSciences Biotech Co Ltd)
90
MultiSciences Biotech Co Ltd ecl western blot analysis kit 93-k820-500
CB1 and TRPV1 protein–protein interaction. (A) Anti-TRPV1 antibody selectivity validation. <t>Western</t> <t>blot</t> detects TRPV1 protein expression with apparent molecular weight in agreement with previous reports. It was selectively suppressed by 74% decline following TRPV1 siRNA transfection of HCEC. (B) CB1-induces decline in TRPV1 phosphorylation status. Western blot <t>analysis</t> of TRPV1 immunopreciptiates using an anti-phospho-threonine antibody revealed that 30 min 10 μM WIN preincubation dampened TRPV1 phosphorylation. Anti-phosphothreonine antibody specificity was validated based on blot with the positive control provided by the commercial source. (C) Anti-CB1 antibody selectivity validation. Western blot detects CB1 protein expression with apparent molecular weight in agreement with previous reports. It was selectively suppressed by 76% decline following CB1 siRNA transfection. (D) TRPV1 complexation with CB1 and TAK1. Co-immunoprecipitation of whole cell lysates obtained with either an anti-TAK1, CB1 or TRPV1 antibody following CAP (10 μM) exposure. Immunocomplexes were probed for TRPV1 content with Western blot analysis using an anti-TRPV1 antibody. (E) CB1 complexation with TRPV1 and TAK1. Co-immunoprecipitation of whole cell lysates obtained with either an anti-TAK1, CB1 or TRPV1 antibody following CAP (10 μM) exposure. Immunocomplexes were probed for CB1 content with Western blot analysis using an anti-CB1 antibody. Antibody selectivity was validated by showing that the IgG immunoprecipitated complexes yielded no immunoreactive bands. Results are expressed as means±SEM (n=3). Differences of p<0.05 were considered as significant.
Ecl Western Blot Analysis Kit 93 K820 500, supplied by MultiSciences Biotech Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ecl western blot analysis kit 93-k820-500/product/MultiSciences Biotech Co Ltd
Average 90 stars, based on 1 article reviews
ecl western blot analysis kit 93-k820-500 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


CB1 and TRPV1 protein–protein interaction. (A) Anti-TRPV1 antibody selectivity validation. Western blot detects TRPV1 protein expression with apparent molecular weight in agreement with previous reports. It was selectively suppressed by 74% decline following TRPV1 siRNA transfection of HCEC. (B) CB1-induces decline in TRPV1 phosphorylation status. Western blot analysis of TRPV1 immunopreciptiates using an anti-phospho-threonine antibody revealed that 30 min 10 μM WIN preincubation dampened TRPV1 phosphorylation. Anti-phosphothreonine antibody specificity was validated based on blot with the positive control provided by the commercial source. (C) Anti-CB1 antibody selectivity validation. Western blot detects CB1 protein expression with apparent molecular weight in agreement with previous reports. It was selectively suppressed by 76% decline following CB1 siRNA transfection. (D) TRPV1 complexation with CB1 and TAK1. Co-immunoprecipitation of whole cell lysates obtained with either an anti-TAK1, CB1 or TRPV1 antibody following CAP (10 μM) exposure. Immunocomplexes were probed for TRPV1 content with Western blot analysis using an anti-TRPV1 antibody. (E) CB1 complexation with TRPV1 and TAK1. Co-immunoprecipitation of whole cell lysates obtained with either an anti-TAK1, CB1 or TRPV1 antibody following CAP (10 μM) exposure. Immunocomplexes were probed for CB1 content with Western blot analysis using an anti-CB1 antibody. Antibody selectivity was validated by showing that the IgG immunoprecipitated complexes yielded no immunoreactive bands. Results are expressed as means±SEM (n=3). Differences of p<0.05 were considered as significant.

Journal: Cellular signalling

Article Title: Cannabinoid receptor 1 suppresses transient receptor potential vanilloid 1-induced inflammatory responses to corneal injury

doi: 10.1016/j.cellsig.2012.10.015

Figure Lengend Snippet: CB1 and TRPV1 protein–protein interaction. (A) Anti-TRPV1 antibody selectivity validation. Western blot detects TRPV1 protein expression with apparent molecular weight in agreement with previous reports. It was selectively suppressed by 74% decline following TRPV1 siRNA transfection of HCEC. (B) CB1-induces decline in TRPV1 phosphorylation status. Western blot analysis of TRPV1 immunopreciptiates using an anti-phospho-threonine antibody revealed that 30 min 10 μM WIN preincubation dampened TRPV1 phosphorylation. Anti-phosphothreonine antibody specificity was validated based on blot with the positive control provided by the commercial source. (C) Anti-CB1 antibody selectivity validation. Western blot detects CB1 protein expression with apparent molecular weight in agreement with previous reports. It was selectively suppressed by 76% decline following CB1 siRNA transfection. (D) TRPV1 complexation with CB1 and TAK1. Co-immunoprecipitation of whole cell lysates obtained with either an anti-TAK1, CB1 or TRPV1 antibody following CAP (10 μM) exposure. Immunocomplexes were probed for TRPV1 content with Western blot analysis using an anti-TRPV1 antibody. (E) CB1 complexation with TRPV1 and TAK1. Co-immunoprecipitation of whole cell lysates obtained with either an anti-TAK1, CB1 or TRPV1 antibody following CAP (10 μM) exposure. Immunocomplexes were probed for CB1 content with Western blot analysis using an anti-CB1 antibody. Antibody selectivity was validated by showing that the IgG immunoprecipitated complexes yielded no immunoreactive bands. Results are expressed as means±SEM (n=3). Differences of p<0.05 were considered as significant.

Article Snippet: The immune reactivity was detected with a Western blot analysis kit (Amersham ECL Plus; GE Healthcare Life Sciences, Piscataway, NJ).

Techniques: Biomarker Discovery, Western Blot, Expressing, Molecular Weight, Transfection, Phospho-proteomics, Positive Control, Immunoprecipitation

Blunting by CB1 activation of TRPV1-induced JNK1 and TAK1 phosphorylation. (A) TRPV1 and CB1 mediated JNK1/2 phosphorylation. Western blot analysis with anti-phospho JNK1/2 antibody reveals increases in JNK1/2 phosphorylation induced by 5 min exposure to CAP (20 μM). Such rises were inhibited by 30 min preexposure to either WIN (10 μM), or CPZ (10 μM). Pre-incubation with AM251 (10 μM), for 30 min prior to WIN (10 μM), exposure suppressed declines in CAP (20 μM)-induced JNK1/2 phosphorylation induced by WIN (10 μM). (B) Dependence of WIN blunting of TRPV1-induced TAK1 phosphorylation on CB1 protein expression. CAP (10 μM)-induced increases in TAK1 phosphorylation at 5 min in cells transduced with scrambled siRNA (left panel) are compared with those in CB1 siRNA transduced cells (right panel). WIN-induced suppression of TRPV1-induced TAK1 phosphorylation was eliminated in CB1 siRNA transduced cells (last lane on the right). The results are shown as means±SEM (n=3, p<0.05).

Journal: Cellular signalling

Article Title: Cannabinoid receptor 1 suppresses transient receptor potential vanilloid 1-induced inflammatory responses to corneal injury

doi: 10.1016/j.cellsig.2012.10.015

Figure Lengend Snippet: Blunting by CB1 activation of TRPV1-induced JNK1 and TAK1 phosphorylation. (A) TRPV1 and CB1 mediated JNK1/2 phosphorylation. Western blot analysis with anti-phospho JNK1/2 antibody reveals increases in JNK1/2 phosphorylation induced by 5 min exposure to CAP (20 μM). Such rises were inhibited by 30 min preexposure to either WIN (10 μM), or CPZ (10 μM). Pre-incubation with AM251 (10 μM), for 30 min prior to WIN (10 μM), exposure suppressed declines in CAP (20 μM)-induced JNK1/2 phosphorylation induced by WIN (10 μM). (B) Dependence of WIN blunting of TRPV1-induced TAK1 phosphorylation on CB1 protein expression. CAP (10 μM)-induced increases in TAK1 phosphorylation at 5 min in cells transduced with scrambled siRNA (left panel) are compared with those in CB1 siRNA transduced cells (right panel). WIN-induced suppression of TRPV1-induced TAK1 phosphorylation was eliminated in CB1 siRNA transduced cells (last lane on the right). The results are shown as means±SEM (n=3, p<0.05).

Article Snippet: The immune reactivity was detected with a Western blot analysis kit (Amersham ECL Plus; GE Healthcare Life Sciences, Piscataway, NJ).

Techniques: Activation Assay, Phospho-proteomics, Western Blot, Incubation, Expressing, Transduction